Genome Assembly and Microsatellite Marker Development Using Illumina and PacBio Sequencing in the Carex pumila (Cyperaceae) from Korea
https://doi.org/10.3390/genes14112063
Kang-Rae Kim,Jeong-Nam Yu,Jeong Min Hong, Sun-Yu Kim andSo Young Park
Genes 14(11), 2063 (2023).
遺伝子の同定による以下の発展
・タンパク質遺伝子
・分子遺伝学的位置
・マイクロサテライトマーカー
Primers for microsatellite markers determined 30 polymorphic markers out of 100.
whole-genome sequencing：870,146,384 reads
total read length： 131,392,103,984 bp.
assembled: from 2941 contigs
genome size: 0.346 Gb
repeats in the genome: 31.0%
heterozygosity range: 0.426 to 0.441%
integrity evaluation of the assembly
: 1481 complete benchmarked universal single-copy orthologs (BUSCO) (91.76%)
protein-coding genes: 23,402